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Microbial isolates from rhizosphere BONCAT-Live. (A) Single cells from salicin and malate gates were sorted on <t>R2A</t> agar and incubated to form colonies. Shown are example plates and identity of selected bacterial colonies based on SSU rRNA amplicon sequencing. (B) SSU rRNA phylogeny of bacterial species for which strains were isolated from salicin and malate BONCAT-Live.
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Microbial isolates from rhizosphere BONCAT-Live. (A) Single cells from salicin and malate gates were sorted on R2A agar and incubated to form colonies. Shown are example plates and identity of selected bacterial colonies based on SSU rRNA amplicon sequencing. (B) SSU rRNA phylogeny of bacterial species for which strains were isolated from salicin and malate BONCAT-Live.

Journal: bioRxiv

Article Title: BONCAT-Live for isolation and cultivation of active environmental microbes

doi: 10.1101/2025.05.14.654084

Figure Lengend Snippet: Microbial isolates from rhizosphere BONCAT-Live. (A) Single cells from salicin and malate gates were sorted on R2A agar and incubated to form colonies. Shown are example plates and identity of selected bacterial colonies based on SSU rRNA amplicon sequencing. (B) SSU rRNA phylogeny of bacterial species for which strains were isolated from salicin and malate BONCAT-Live.

Article Snippet: The strains were maintained individually in R2A medium (Thermo Scientific) at 25°C.

Techniques: Incubation, Amplification, Sequencing, Isolation